Journal: EMBO Reports

Article Title: Global and precise identification of functional miRNA targets in mESCs by integrative analysis

doi: 10.15252/embr.202254762

Figure Lengend Snippet: Immunoblot analysis of DROSHA, DICER, AGO1, and AGO2 in WT and their respective KO mESC lines. TUBULIN was used as a loading control. Representative blot of three independent experiments is shown. PCA of gene expression as measured by RNA sequencing in miRNA _KO and WT samples. Biological replicates are indicated with corresponding colors. MA plots of the DGE analysis in miRNA _KO mutants versus WT. Significant up‐ and downregulated genes are colored in red and blue, respectively. Overlap of up‐ (left) and downregulated (right) genes in miRNA _KO mESCs. Comparison of miRNA loading for small RNA sequencing (Input) versus Argonaute2 & 1 (AGOs) RNA immunoprecipitation (RIP) and sequencing for all miRNAs. MiRNAs that show statistically significant difference between the two measured (adjusted P ‐value < 0.1) are highlighted. Data information: In (C and E), significance in differential analysis was determined using an adjusted P ‐value threshold obtained from DESeq2 of 0.1. In (D), an adjusted P ‐value threshold of 0.2 was applied. Source data are available online for this figure.

Article Snippet: pX458‐sgRNA_ Ago1 _2 , Addgene , #73534.

Techniques: Western Blot, Control, Gene Expression, RNA Sequencing, Comparison, RNA Immunoprecipitation, Sequencing

Journal: EMBO Reports

Article Title: Global and precise identification of functional miRNA targets in mESCs by integrative analysis

doi: 10.15252/embr.202254762

Figure Lengend Snippet:

Article Snippet: pX458‐sgRNA_ Ago1 _2 , Addgene , #73534.

Techniques: Recombinant